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ICAR JRF Aquatic Animal Health Management 29 Jun 2024Shift 1
MCQ (Single Correct) +4 −1
Strand displacement activity of DNA polymerase is the characteristic principle of which diagnostic technique
  • Loop-Mediated Isothermal Amplification (LAMP):
  • LAMP: An isothermal nucleic acid amplification technique operating at a constant temperature ($60-65^\circ\text{C}$).
  • It relies on a specialized DNA polymerase (typically Bst DNA Polymerase Large Fragment) possessing extraordinary strand displacement activity coupled with 4–6 specific primers, forming characteristic loop-stem amplicons without requiring thermal denaturation cycles.
  • Correct Option: Option 2 (LAMP).
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