LAMP: An isothermal nucleic acid amplification technique operating at a constant temperature ($60-65^\circ\text{C}$).
It relies on a specialized DNA polymerase (typically Bst DNA Polymerase Large Fragment) possessing extraordinary strand displacement activity coupled with 4–6 specific primers, forming characteristic loop-stem amplicons without requiring thermal denaturation cycles.
Correct Option: Option 2 (LAMP).
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ICAR JRF Aquatic Animal Health Management 29 Jun 2024Shift 1
MCQ (Single Correct)+4−1
Which of the following is a non-radioactive label used in situ hybridisation?
In In Situ Hybridization (ISH), Digoxigenin (DIG) (a plant steroid hapten isolated from Digitalis purpurea) is widely used as a sensitive, non-radioactive nucleotide label.
DIG-labeled nucleic acid probes hybridize to target pathogen DNA/RNA in tissue sections and are detected via anti-DIG antibody fragments conjugated to alkaline phosphatase.
Correct Option: Option 4 (Digoxigenin).
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