• Definition of Isoschizomers: Restriction endonucleases that recognize the identical target nucleotide sequence.
• Hpall and Mspl: Both enzymes recognize the tetranucleotide palindromic sequence $5'\text{-CCGG-3'}$. They differ in methylation sensitivity: HpaII is inhibited if the internal cytosine is methylated ($5'\text{-C}^m\text{CGG-3'}$), whereas MspI cleaves whether the internal cytosine is methylated or unmethylated, making them indispensable tools for studying DNA methylation.
• Neoschizomers: Enzymes that recognize the same sequence but cleave at different positions.

Correct option: (2) Hpall and Mspl

• Southern Blotting: Developed by British biologist Edwin Southern (1975) to identify and detect specific target DNA molecules.
• Process: DNA is digested with restriction endonucleases, resolved by agarose gel electrophoresis, capillary-transferred to a nitrocellulose or nylon membrane, and hybridized with a labeled complementary DNA/RNA probe.
• Distinctions: Northern blotting detects specific RNA; Western blotting detects specific proteins; Eastern blotting detects post-translational modifications.

Correct option: (1) Southem blotting