3
Given below are two statements
Statement (I) : CDNA clones are generated from processed mRNA which lack introns and other sequences surrounding the
gene
Statement (II) : CDNA libraries are used when the gene of interest is expressed in a particular tissue or cell type
In light of the above statements, choose the most appropriate answer from the options given below.
EXPLANATION
Recombinant DNA Technology, Cloning Vectors,
- Statement (I) is True: Complementary DNA (cDNA) clones are synthesized from mature, spliced eukaryotic mRNA using reverse transcriptase; they represent only the expressed exonic coding regions and lack non-coding introns, 5' upstream promoters, and intergenic regions.
- Statement (II) is True: cDNA libraries capture the active transcriptome of a specific tissue, organ, or developmental stage, facilitating the cloning and functional expression of tissue-specific genes.
- Conclusion: Both Statement (I) and Statement (II) are true.
Correct option: (1) Both Statement (I) and Statement (II) are true.
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