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Which one of the following reagent is used for detection of presence of detergent in milk
EXPLANATION
Chemical Detection of Milk Adulterants
- Detection of Synthetic Detergent Adulteration in Milk:
- Methylene Blue Extraction Assay:
- Detergents contain anionic surfactants (e.g., linear alkylbenzene sulfonates, LAS).
- Anionic surfactants form an ion-pair complex with the cationic dye Methylene Blue, which is extracted into chloroform, imparting an intense blue color to the lower chloroform layer.
- Conclusion: Methylene Blue (Option 4).
Correct option: (4) Methylene Blue
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