8
Which statements are true with respect to non-protein nitrogen (NPN) in milk
(A) . Nitrogen soluble in 12% trichloroacetic acid (TCA) is referred as NPN
(B) . Nitrogen soluble at pH 4.6 is referred as. NPN
(C) . Nitrogen insoluble in 12% trichloroacetic acid (TCA) is referred as NPN
(D) . Nitrogen insoluble at pH 4.6 is referred as NPN
Choose the correct answer from the options given below:
EXPLANATION
Casein Fractionation
- Analytical Fractionation of Non-Protein Nitrogen (NPN) in Milk:
- Standard Definition (Rowland Fractionation Protocol):
- Non-Protein Nitrogen (NPN) is chemically defined as the nitrogen fraction that remains soluble in 12% trichloroacetic acid (TCA) solution.
- 12% TCA quantitatively precipitates all proteins (caseins and native/denatured whey proteins), leaving only low-molecular-weight nitrogenous compounds (urea, creatine, creatinine, free amino acids, peptides) in the filtrate.
- Conclusion: (A) only (Option 2).
Correct option: (2) (A) only,
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