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ICAR JRF Dairy Chemistry 29 Jun 2024Shift 1
MCQ (Single Correct) +4 −1
Why is it generally preferable to use absorbance as a measure of absorption rather than % transmittance?
  • Absorbance vs. Percent Transmittance in Spectrophotometry:
  • Beer-Lambert Law Linearity:
$$A = -\log_{10}(T) = \epsilon c l$$
  • Absorbance ($A$) is directly proportional to analyte concentration ($c$), yielding linear calibration curves.
  • Percent Transmittance ($\%T = 100 \times 10^{-A}$) exhibits a non-linear exponential decay with concentration, complicating quantitative determinations.
  • Conclusion: Because absorbance is proportional to the concentration of the analyte, whereas %T is not (Option 3).

Correct option: (3) Because absorbance is proportional to the concentration of the analyte, whereas %T is not

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