The formula used in Thin Layer Chromatography... | Dairy Chemistry

ICAR JRF Dairy Chemistry 29 Jun 2024Shift 1

MCQ (Single Correct) +4 −1

The formula used in Thin Layer Chromatography for the calculation of Rf value of an individual compound is

Distance compound has moved from origin/Distance of solvent front from origin Correct Distance of solvent front from origin/Distance compound has moved from origin Time taken by solvent front to move to a particular position from origin/Time taken by the compound to move to a particular position from origin Time taken by the compound to move to a particular position from origin/Time taken by solvent front to move to a particular position from origin

Calculation of Retention Factor ($R_f$) in TLC:
Mathematical Definition:

$$R_f = \frac{\text{Distance migrated by the individual compound from origin}}{\text{Distance migrated by the solvent front from origin}}$$

dimensionless chromatographic ratio characterizing solute migration relative to mobile phase travel ($0 \le R_f \le 1$).
Conclusion: Distance compound has moved from origin/Distance of solvent front from origin (Option 1).

Correct option: (1) Distance compound has moved from origin/Distance of solvent front from origin

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