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ICAR JRF Dairy Chemistry 29 Jun 2024Shift 1
MCQ (Single Correct) +4 −1
Polymerization of acrylamide and bisacrylamide monomers in SDS PAGE is induced by
  • Free Radical Polymerization in SDS-PAGE Gel Casting:
  • Initiator Chemistry:
  • Polyacrylamide gel casting uses acrylamide monomer and N,N'-methylenebisacrylamide crosslinker.
  • Polymerization is initiated by free sulfate radicals produced by Ammonium persulfate (APS), catalyzed by TEMED (tetramethylethylenediamine).
  • Conclusion: Ammonium persulfate (Option 1).

Correct option: (1) Ammonium persulfate

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