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Which of the following is not a next generation sequencing platform
EXPLANATION
Next-Generation Sequencing Platforms & Riboty
- Generations of Sequencing Technologies:
- First-Generation Sequencing:
- Sanger Sequencing: Dideoxynucleotide chain termination using capillary electrophoresis. It is NOT a Next-Generation Sequencing (NGS) platform.
- Next-Generation Sequencing (NGS / 2nd Generation):
- Roche 454: Pyrosequencing on beads.
- Ion Torrent / Ion Proton: Semiconductor sequencing detecting released $H^+$ ions.
- Illumina: Reversible dye-terminator sequencing by synthesis.
- Third-Generation Sequencing (Long Reads):
- Oxford Nanopore Technologies (ONT): Direct electrical current disruption through protein nanopores.
- PacBio SMRT: Single-molecule real-time sequencing.
- nalysis:
- (Sanger sequencer): Correct (first-generation, not NGS).
Correct option: (1) Sanger sequencer
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