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The restriction enzyme that makes blunt cuts in DNA is
EXPLANATION
Restriction Endonucleases, Modifying Enzymes
- Blunt-End Cleavage by EcoRV:
- Restriction endonucleases cleave palindromic recognition sequences:
- EcoRV (from E. coli): Cleaves at $5'\text{-GAT}^\downarrow\text{ATC-3'}$, producing blunt (flush) ends:
- EcoRI ($5'\text{-G}^\downarrow\text{AATTC-3'}$), BamHI ($5'\text{-G}^\downarrow\text{GATCC-3'}$), and HindIII ($5'\text{-A}^\downarrow\text{AGCTT-3'}$) produce staggered sticky (cohesive) $5'$ overhangs.
- Correct Option: Option 4 (EcoRV).
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