A Taqman probe is related to | Fish Biotechnology

ICAR JRF Fish Biotechnology 29 Jun 2024Shift 1

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A Taqman probe is related to__

genotyping by real-time PCR Correct genotyping by Southern hybridisation genotyping by high resolution melting curve genotyping by PCR and fragment analysis

TaqMan Probes in Quantitative Real-Time PCR:
TaqMan Probe: An oligonucleotide probe dual-labeled with a $5'$ fluorescent reporter dye (e.g., FAM) and a $3'$ quencher dye (e.g., TAMRA).
During real-time PCR, Taq DNA polymerase's $5' \to 3'$ exonuclease activity cleaves the hybridized probe, releasing the reporter from quenching and generating fluorescence proportional to accumulated amplicon. It is the gold standard for genotyping and SNP detection in real-time PCR.
Correct Option: Option 1 (genotyping by real-time PCR).

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