15
Which of these is not true for 16S metagenomic sequencing by NGS?
EXPLANATION
Metagenomics of Aquatic Ecosystems
- 16S Metagenomic Amplicon Sequencing:
- 16S rRNA Gene Sequencing: Amplifies specific hypervariable regions (typically V3 and V4) using universal primers to profile microbial taxonomic composition and relative abundance in environmental samples.
- It is an amplicon marker-gene assay and does not sequence the full metagenome or yield novel functional genes. Discovering novel metabolic genes requires shotgun whole-metagenome sequencing.
- Correct Option: Option 2 (It yields novel genes from the sequenced microbes).
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