7
Complementary DNA (cDNA) is
EXPLANATION
High-Throughput Sequencing
- Complementary DNA (cDNA):
- cDNA is synthetic DNA synthesized in vitro from a single-stranded mature messenger RNA (mRNA) template catalyzed by the enzyme reverse transcriptase (RNA-dependent DNA polymerase), followed by second-strand DNA synthesis.
- Because mature eukaryotic mRNA has already undergone post-transcriptional splicing, cDNA contains strictly the continuous coding exons of genes without non-coding introns.
- nalysis:
- (mRNA reverse transcribed to DNA): Correct.
Correct option: (3) mRNA reverse transcribed to DNA
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