5
Which of the following is not a method of DNA sequencing?
EXPLANATION
Nutrigenomics, Transcriptional Regulation, SR
- Methods of Nucleic Acid and Protein Sequencing:
- DNA Sequencing Methods:
- Maxam-Gilbert Method: Chemical cleavage method using dimethyl sulfate and hydrazine.
- Sanger Sequencing: Dideoxy chain termination method.
- Pyrosequencing: Sequencing-by-synthesis based on detection of released pyrophosphate ($PPi$).
- Illumina (Solexa): Next-generation reversible dye-terminator sequencing.
- Edman Degradation: A classic biochemical method for sequencing amino acids in a peptide/protein from the N-terminus, using phenylisothiocyanate (PITC). It is NOT a DNA sequencing method.
- nalysis:
- (Edman degradation): Correct (it sequences proteins, not DNA).
Correct option: (1) Edaman degradation
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