In teleost ovarian histology, maturity stages progress through primary growth, cortical alveoli, vitellogenesis, maturation, ovulation, and post-spawning.
Hydrated Oocytes: During Final Oocyte Maturation (FOM), oocytes take up water rapidly, causing coalescence of yolk spheres, clearing of cytoplasm, and massive volumetric swelling (hydration). The presence of fully hydrated, translucent oocytes is the definitive, pathognomonic histological hallmark of an actively spawning (running-ripe) ovary immediately prior to ovulation.
Post-ovulatory follicles (POFs) indicate past spawning that has already concluded.
Correct Option: Option 2 (Hydrated oocytes).
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