Q.2
Make the correct sequence of steps involved in marker assisted breeding in flower crops.
(A) . Correlation of molecular markers with morphological traits.
(B) . Scoring for markers for determination of polymorphism between parents and Fz progeny.
(C) . Polymerase chain reaction and gel electrophorisis
(). Development of breeding populations like F progeny.
(E) . DNA isolation and purification
(F). Selection of divergent parents.
Choose the correct answer from the options given below:
- Logical Sequence in Marker-Assisted Breeding (MAB):
- (F) Selection of divergent parents: Identifying genetically and morphologically contrasting parental lines for target traits.
- Development of breeding populations like $F_2$ progeny: Hybridizing selected parents and selfing $F_1$ to generate segregating $F_2$ populations.
- DNA isolation and purification: Extracting high-quality genomic DNA from parents and segregating progenies.
- Polymerase chain reaction and gel electrophoresis: Amplifying polymorphic DNA sequences with primers and separating amplicon bands.
- Scoring for markers for determination of polymorphism: Genotyping allelic banding patterns across individuals.
6. (A) Correlation of molecular markers with morphological traits: Conducting linkage and QTL mapping to associate molecular markers with target phenotypes.- Correct Sequence: (F), (D), (E), (C), (B) and (A), matching Option 3.
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