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ICAR JRF Food Processing Technology 29 Jun 2024Shift 1
MCQ (Single Correct)
+4
−1
Methylene of Blue Reduction Test (MBRT) is conducted to know
EXPLANATION
Indicator Organisms of Food Sanitation
- Methylene Blue Reduction Test (MBRT) in Milk Quality Control:
- Biochemical Principle:
- Dye reduction test measuring the metabolic activity and approximate bacterial load of raw milk.
- Proliferating bacteria consume dissolved oxygen, lowering redox potential ($E_h$), reducing the blue methylene blue dye to colorless leuko-methylene blue.
- Standard Grading:
- $\gt 5\text{ hours}$: Very Good ($\lt 2 \times 10^5\text{ CFU/mL}$).
- $3 - 4\text{ hours}$: Good ($2 \times 10^5 - 10^6\text{ CFU/mL}$).
- $1 - 2\text{ hours}$: Fair ($10^6 - 10^7\text{ CFU/mL}$).
- $\lt 30\text{ minutes}$: Poor ($\gt 10^7\text{ CFU/mL}$).
- Conclusion: Indication of bacterial load in milk (Option 1).
Correct option: (1) Indication of bacterial load in milk
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