Basic Microbiological Techniques Practice Questions (Q16-20) - Microbiology

ICAR JRF Microbiology 29 Jun 2024Shift 1

Assertion & Reason +4 −1

Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).

Assertion (A) : Sulphur-oxidising bacteria also have a role to play in the coal mining industry.

Reason (R) : These microbes carry out biodesulphurisation of coal to remove the sulphur before combustion.

In light of the above statements, choose the correct answer from the options given below.

Both (A) and (R) are true and (R) is the correct explanation of (A) Both (A) and (R) are true but (R) is NOT the correct explanation of (A). (A) is true but (R) is false (A) is false but (R) is true.

ICAR JRF Microbiology 29 Jun 2024Shift 1

MCQ (Single Correct) +4 −1

Read the following statements regarding gold biomining

(A) . Gold is typically present in nature in deposits associated with minerals containing arsenic (As) and FeSz (8). Acidithiobacillus ferrooxidans and related bacteria can leach the arsenopyrite minerals, releasing the trapped Au

(C) . Gold leaching is done by the huge dump method, with about 90% gold recovery.

(D) . The potentially toxic Arsenic and Cyanide residues from the mining process are removed in the gold-leaching bioreactor.

Choose the correct answer from the options given below:

(A), (6) and (C) only. (B) and (D) only. (A), (B) and (0) only, (A) and (D) only.

ICAR JRF Microbiology 29 Jun 2024Shift 1

MCQ (Single Correct) +4 −1

Read the following statements regarding use of microarrays for the analysis of microbial diversity

(A) . Itcan be used for phylogenetic and gene expression analysis.

(B) . Phylochips, a microarray designed for biodiversity studies, can be used for screening of microbial communities for specific groups of prokaryotes.

(C) . For phylochips microarray analysis, genes encoding small subunit of rRNA need to be amplified by PCR followed by cloning and sequencing of the targeted gene

(D) . Microarray can also detect genes encoding functions of biogeochemical significance.

(E) . Totally unrelated genes may yield false positive results if they are sufficiently complementary to the probe to cause hybridization.

Choose the correct answer from the options given below:

(A), (B) and (C) only. (A), (B) and (E) only. (6), (C) and (D) only. (A), (B), (D) and (E) only.

ICAR JRF Microbiology 29 Jun 2024Shift 1

Statement Based +4 −1

Given below are two statements

Statement (I) : Genes such as those encoding rRNAs that have changed in sequence over time as species have diverged are called orthologs.

Statement (II) : Organisms that share the same or very closely related orthologous genes are called a phylotype.

In light of the above statements, choose the most appropriate answer from the options given below.

Both Statement (I) and Statement (II) are true. Both Statement (I) and Statement (II) are false. Statement (I) is true but Statement (II) is false. Statement (I) is false but Statement ({I) is true.

ICAR JRF Microbiology 29 Jun 2024Shift 1

Assertion & Reason +4 −1

Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).

Assertion (A) : Green fluorescent protein (gfp) gene based tracking is not suitable for methanogenic bacteria

Reason (R) : Methanogens are strictly anaerobic in nature and can't produce sufficient energy for fluorescent production at the detectable level

In light of the above statements, choose the correct answer from the options given below.

Both (A) and (R) are true and (R) is the correct explanation of (A) Both (A) and (R) are true but (R) is NOT the correct explanation of (A). (A) is true but (R) is false. (A) is false but (R) is true.