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Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?
EXPLANATION
Molecular Genetics of Immunity, R-Genes, SAR/
- Principle of Antisense Oligonucleotides: An antisense molecule is an oligonucleotide designed to be strictly complementary in sequence and antiparallel in polarity to a targeted sense mRNA or coding DNA sequence, inhibiting translation or transcription through hybrid formation.
- Sequence Pairing: For the target sequence $5'\text{-ATTAAGGC-}3'$:
- The complementary Watson-Crick base pairing gives: $A \rightarrow T$ (or $U$ in RNA), $T \rightarrow A$, $G \rightarrow C$, and $C \rightarrow G$.
- Matching base-by-base yields the antiparallel complementary strand: $3'\text{-TAATTCCG-}5'$ (or $5'\text{-TAATTCCG-}3'$ when presented in direct alignment as an antisense sequence in the question).
- Conclusion: TAATTCCG represents the designated antisense molecule (Option 4).
Correct option: (4) TAATTCCG
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