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ICAR JRF Plant Pathology 29 Jun 2024Shift 1
MCQ (Single Correct) +4 −1
Given the DNA sequence ATTAAGGC, which sequence would be used by researchers as an antisense molecule?
  • Principle of Antisense Oligonucleotides: An antisense molecule is an oligonucleotide designed to be strictly complementary in sequence and antiparallel in polarity to a targeted sense mRNA or coding DNA sequence, inhibiting translation or transcription through hybrid formation.
  • Sequence Pairing: For the target sequence $5'\text{-ATTAAGGC-}3'$:
  • The complementary Watson-Crick base pairing gives: $A \rightarrow T$ (or $U$ in RNA), $T \rightarrow A$, $G \rightarrow C$, and $C \rightarrow G$.
  • Matching base-by-base yields the antiparallel complementary strand: $3'\text{-TAATTCCG-}5'$ (or $5'\text{-TAATTCCG-}3'$ when presented in direct alignment as an antisense sequence in the question).
  • Conclusion: TAATTCCG represents the designated antisense molecule (Option 4).

Correct option: (4) TAATTCCG

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