13
Macromolecules like proteins can be purified and separated using
(A) . Decantation
(B) . Chromatography
(C) . iso-electric precipitation
(D) . GeLfitration
Choose the correct answer from the options given below:
EXPLANATION
Cold Chain Management & Modified Atmosphere
- Purification and Separation of Macromolecular Proteins:
- Specialized biochemical separation techniques for proteins include:
- Chromatography: Ion-exchange, affinity, and hydrophobic interaction chromatography exploit specific surface charges and binding affinities.
- Iso-electric precipitation: Proteins carry zero net charge at their isoelectric point ($pI$), minimizing electrostatic repulsion and causing selective precipitation.
- Gel filtration: Size-exclusion chromatography separates protein molecules based on hydrodynamic molecular weight through porous dextran/agarose beads.
- (A) Decantation is a coarse gravity separation of macroscopic sediments, unsuitable for molecular-level protein fractionation.
- Conclusion: (B), (C) and (D) only (Option 4).
Correct option: (4) (B), (C) and (D) only.
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