Diapause Termination & Cold Storage of Silkworm Eggs:
Bivoltine silkworm eggs enter embryonic diapause at the gastrula stage, regulated by diapause hormone (DH).
To break diapause artificially by cold preservation, diapausing eggs are conserved at chilling temperatures of $5^\circ\text{C}$ ($2.5 - 5^\circ\text{C}$) under $75 - 80\%$ relative humidity.
Diapause termination commences after $50 - 60\text{ days}$ of chilling at $5^\circ\text{C}$, enabling synchronized reactivation of embryonic development upon subsequent incubation at $25^\circ\text{C}$.
Correct Option: Option 4 (5 degree centigrade).
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