Q.2
Given below are two statements, one is labelled as Assertion (A) and other one labelled as Reason (R).
Assertion (A) : Dehydrogenase activity reflects the total range of the oxidative activity of soil microorganisms and may be
considered a good indicator of the oxidative metabolism in soils.
Reason (R) : Dehydrogenase oxidizes soil organic matter by transferring protons and electrons from organic substrates to
inorganic acceptors.
In light of the above statements, choose the correct answer from the options given below.
- Assertion (A) is true: Soil dehydrogenase enzyme activity exists strictly within intact viable microbial cells and serves as an established index of total oxidative microbiological activity and biological respiration in soils.
- Reason (R) is true & correct explanation: Dehydrogenases catalyze the oxidation of soil organic matter by transferring hydrogen protons ($H^+$) and electrons from organic electron donors to specific intracellular electron acceptors (NAD/NADP, cytochromes, and tetrazolium salts in assays). This electron transport mechanism directly accounts for why dehydrogenase activity reflects oxidative metabolism.
- Conclusion: Both (A) and (R) are true and (R) is the correct explanation of (A). (Correct answer: Option 1)
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