5
Lymphocytes in whole blood can be stimulated with
EXPLANATION
Primary Cell Culture vs Established Cell Line
- Lymphocyte Blastogenesis and Mitogens:
- In whole blood lymphocyte cultures for cytogenetic karyotyping or cell-mediated immune assays:
- Phytohemagglutinin (PHA), a plant lectin extracted from the red kidney bean (Phaseolus vulgaris), binds to surface carbohydrate receptors on T-lymphocytes, acting as a potent polyclonal mitogen that stimulates blast transformation, DNA synthesis, and mitotic cell division.
- nalysis:
- (Phytohemagglutinin): Correct mitogen.
Correct option: (2) Phytohemagglutinin.
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