• Generation of Transgenic Livestock and Rodents:
• Pronuclear DNA Microinjection (Gordon & Ruddle, 1980):
• Linearized recombinant transgene constructs are injected directly into the male pronucleus of freshly fertilized embryos at the One-Cell Stage (zygote).
• Performing microinjection at the single-cell stage prior to the first mitotic cleavage ensures that the integrated transgene is replicated and passed into every embryonic cell lineage, minimizing germline mosaicism.
• nalysis:
• (One-cell-stage): Correct developmental stage.

Correct option: (1) One-cell-stage.