Q66: Homopolymers can be added to DNA strands by using - Molecular Biology and Recombinant DNA Methods - Veterinary Biochemistry- ICAR JRF/SRF 2024 | Agrigyan

• Homopolymer Tailing in Recombinant DNA Technology:
• Terminal Deoxynucleotidyl Transferase (TdT): A template-independent DNA polymerase that adds successive deoxynucleotides to the $3'\text{-OH}$ end of DNA.
• When supplied with a single dNTP (e.g., dGTP or dCTP), TdT synthesizes a continuous homopolymeric tail (e.g., poly-dG on vector and poly-dC on insert cDNA), allowing complementary annealing and cohesive cloning without restriction enzyme cleavage.
• nalysis:
• (Terminal deoxynucleotidyltransferase): Correct enzyme.

Correct option: (2) Terminal deoxynucleotidyltransferase