Spontaneous and induced mutations arise through replication slippage, unequal crossing over, or mutagens causing the insertion or deletion of one or a few nucleotides (indels).
If the indel length is not a multiple of three, it causes a catastrophic frameshift mutation, completely altering the downstream amino acid reading frame.
nalysis:
(Insertion or deletion of one or a few nucleotides): Correct source of genetic mutation.
Correct option: (2) Insertion or deletion of one or a few nucleotides.
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2
ICAR JRF Veterinary Biotechnology 29 Jun 2024Shift 1
MCQ (Single Correct)+4−1
Which of the following is not a DNA repair system?
Mismatch Repair (MMR): Corrects post-replicative base mispairings and insertion/deletion loops.
Non-Homologous End-Joining (NHEJ): Directly ligates double-strand breaks without a homologous template.
Branch Migration: A physical thermodynamic process where homologous strands exchange pairing partners during meiotic or mitotic homologous recombination (Holliday junction movement); it is NOT a DNA repair system.
nalysis:
(Branch migration): Correct (not a repair system).
Correct option: (4) Branch migration
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3
ICAR JRF Veterinary Biotechnology 29 Jun 2024Shift 1
MCQ (Single Correct)+4−1
Chemicals such as add alkyl groups to nucleotides in DNA molecules to cause mutation
Alkylating Agents: Ethylmethanesulfonate (EMS) is a powerful chemical mutagen that transfers ethyl alkyl groups to nitrogenous bases in DNA molecules.
Mechanism of Mutagenesis: EMS primarily ethylates the oxygen at position 6 of guanine, forming $O^6$-ethylguanine. During DNA replication, $O^6$-ethylguanine pairs with thymine instead of cytosine, producing characteristic $\text{G:C} \to \text{A:T}$ transition mutations.
Other Mutagens: Ethidium bromide is an intercalating agent, nitrous acid is a deaminating agent, and 5-bromouracil is a thymine base analog.
Correct option: (2) Ethylmethane sulfonate.
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