Q.3
Match List with List-II
Choose the correct answer from the options given below:
Choose the correct answer from the options given below :
|
List - I
Type of H and N antigen
|
List - II
Related to the virus
|
||
|---|---|---|---|
| A | H7N7 H7N7 | I | Equine influenza virus 2 Equine influenza virus 2 |
| B | H3N8 H3N8 | II | Avian influenza Avian influenza |
| C | H1N1 H1N1 | III | Equine influenza virus 1 Equine influenza virus 1 |
| D | H5N1 H5N1 | IV | Swine influenza Swine influenza |
- Matching Viral Diagnostic Assays with Their Principles:
- Hemagglutination Assay (HA) $\longrightarrow$ (III) Surface glycoprotein binding: Measures binding of viral surface hemagglutinin (e.g., in Newcastle disease virus, Influenza) to sialic acid receptors on RBCs.
- Enzyme-Linked Immunosorbent Assay (ELISA) $\longrightarrow$ (I) Enzyme-conjugated antibody detection: Utilizes antibody-antigen complexes quantified via colorimetric substrate conversion.
- Polymerase Chain Reaction (PCR) $\longrightarrow$ (IV) Exponential nucleic acid amplification: Enzymatic in vitro amplification of specific viral DNA or cDNA fragments using thermostable DNA polymerase.
- Virus Neutralization Test (VNT) $\longrightarrow$ (II) Specific antibody inhibition of viral infectivity in cell culture: Benchmark gold-standard serological assay assessing antibody capacity to prevent viral cytopathic effects (CPE).
- Conclusion: (A) - (III), (B) - (I), (C) - (IV), (D) - (II) (Option 1).
Correct option: (1) (A) - (ill), (B) - (1), (C) - (IV), (B) - (I)
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