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ICAR JRF Veterinary Microbiology 29 Jun 2024Shift 1
MCQ (Single Correct)
+4
−1
Incubation conditions for the growth of dermatophytes are:
EXPLANATION
Dermatophytes
- Culture Conditions for Dermatophytes: Standard diagnostic cultivation of dermatophytes (Microsporum, Trichophyton, and Epidermophyton) from hair, skin scrapings, and crusts utilizes Sabouraud Dextrose Agar (SDA) with chloramphenicol and cycloheximide (actidione).
- Incubation Parameters: The inoculated slants must be incubated at $25^\circ\text{C}$ (room temperature) for 2 to 4 weeks.
- Biological Basis: Dermatophytes are slow-growing filamentous moulds that replicate optimally between $25\text{--}30^\circ\text{C}$. Bacterial contaminants outgrow them at $37^\circ\text{C}$, and colonies require at least 10–21 days to develop characteristic macroconidia, microconidia, and pigment production.
Correct option: (4) 25°C for 2-4 weeks
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