Reverse primer for a gene can be a part of the sequence of | Agrigyan

Q.67

Aquaculture- ICAR JRF/SRF 2024

MCQ (Single Correct) +4 −1

Reverse primer for a gene can be a part of the sequence of

5' end of cDNA 5! end of mRNA 3! end of CDNA Correct 3! end of MRNA

PCR Primer Design for cDNA:
In molecular biology and RT-PCR:
The Forward Primer hybridizes to the complementary anti-sense strand, matching the sequence at the $5'$ end of the coding cDNA.
The Reverse Primer hybridizes to the sense strand and is complementary to the sequence at the $3'$ end of the cDNA coding sequence.
Correct Option: Option 3 (3' end of cDNA).

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