Q.1
Veterinary Biotechnology- ICAR JRF/SRF 2024 (Q.113)
MCQ (Single Correct) +4 −1

Which of the following steps is NOT an esstential process during cell cryopreservation?

Veterinary Biotechnology Subjects

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General Agriculture

6 Topics
Agronomy, Tillage & Field Crop Production Agricultural Entomology, Nematology & Crop Protection Soil Science, Plant Nutrition & Reclamation Cell Biology, Plant Physiology & Biochemistry Agricultural Meteorology & Climate Science Crop Genetics, Plant Breeding & IPR

Biomolecules and their Interaction Relevant to Biology

4 Topics
Biophysical Principles of Biomolecular Conformation: Ramachandran Plot & Thermodynamic Stability Nucleic Acid Structures (A/B/Z-DNA, Quadruplexes) & Sequence-Specific Protein-DNA Interactions Protein Folding Dynamics, Chaperones, Allosteric Transitions & Ligand-Binding Kinetics Biophysical Analysis of Biomolecules: Circular Dichroism, X-Ray Crystallography & NMR

Cellular Basis of Animal Physiology

2 Topics
Cellular Ultrastructure, Nucleolus, Membrane Transport & Glycocalyx Body Fluid Compartments (ICF vs ECF), Osmolality & Starling Forces

Blood and Circulation

1 Topic
Hematopoiesis, Erythropoiesis (Liver, Spleen, Bone Marrow) & Blood Indices

Cell Biology

4 Topics
Eukaryotic Cell Cycle Regulation: Cyclins, CDKs, Checkpoints (G1/S, G2/M) & Senescence Structural & Functional Organization of Intracellular Organelles (Peroxisomes, Mitochondria, Golgi) Cytoskeletal Dynamics: Microtubules, Microfilaments, Intermediate Filaments & Motor Proteins Membrane Ultrastructure, Lipid Rafts & Macromolecular Translocation across Membranes

Chemistry of Carbohydrates and Lipids

2 Topics
Monosaccharides, Oligosaccharides, Stereochemistry & Reducing Sugar Dynamics Fatty Acid Classification, Saturated vs Unsaturated & Trans-Fatty Acids

Chemistry of Amino Acids, Proteins & Nucleic Acids

1 Topic
Amino Acid Classification, Physicochemical Properties, Zwitterions & Isoelectric Point

Fundamental Cellular Processes

6 Topics
Eukaryotic Transcription Factors, Enhancers, Riboswitches & RNA Polymerase I/II/III Machinery DNA Replication Fidelity, Fork Dynamics, Telomerases & Cre-Lox / Site-Specific Recombination DNA Repair Pathways: Base/Nucleotide Excision, Non-Homologous End Joining & Homologous Recombination Translation Mechanisms, Ribosome Subunits, Polysome Profiling & Translational Ribosomal Stalling Post-Transcriptional Processing: Splicing, Alternative Splicing, Capping, Polyadenylation & RNA Editing Epigenetic Gene Regulation: DNA Methylation, Histone Code & Non-Coding RNAs (miRNA, siRNA, lncRNA)

Respiration

1 Topic
Pulmonary Gas Exchange, Partial Pressures (Venous pO2 40 mmHg) & V/Q Matching

Cell Communication and Cell Signalling

4 Topics
Nitric Oxide (NO) - Guanylyl Cyclase (sGC) - cGMP Signalling Axis & Vascular Regulation G-Protein Coupled Receptors (GPCR), Adenylyl Cyclase, cAMP, PKA & Phospholipase C Cascades Receptor Tyrosine Kinases (RTK), MAPK/ERK, PI3K/Akt Pathways & Growth Factor Signalling Cancer Biology: Oncogenes, Tumor Suppressors (p53, Rb), Angiogenesis & Metastatic Cascade

Enzymology

4 Topics
Allosteric Regulation, Covalent Modification, Proenzymes (Zymogens) & Isoenzymes Enzyme Classification (EC System 1-7), Nomenclature & Coenzymes/Cofactors Enzyme Inhibition: Competitive, Non-Competitive, Uncompetitive & Irreversible Enzyme Kinetics: Michaelis-Menten Model, Lineweaver-Burk Plot, Km & Vmax

Excretion

2 Topics
Renal Functional Anatomy, Glomerular Filtration Rate (GFR) & Renal Clearance Endocrine Regulation of Renal Function: Aldosterone (Distal Tubule), ADH & ANP

Bioenergetics and Intermediary Metabolism

1 Topic
Tricarboxylic Acid (TCA/Krebs) Cycle, Amphibolic Nature & Anaplerosis

Digestion

2 Topics
Ruminant Pre-Gastric Fermentative Digestion: Rumen Ecology, Holotrichs & VFAs Nutrient Absorption Mechanisms across Rumen, Small Intestine & Large Intestine

Molecular Biology and Recombinant DNA Methods

5 Topics
Molecular Cloning Strategies: Restriction-Modification, Linkers/Adaptors & Directional Ligation Genomic & cDNA Library Construction, Screening & Ribosome-Bound Transcriptome Probing Next-Generation Sequencing (NGS): Illumina Sequencing-by-Synthesis, PacBio & Oxford Nanopore Cloning & Expression Vectors: Plasmids, Bacteriophages, Cosmids, BACs, YACs & Expression Hosts Nucleic Acid Blotting (Southern, Northern), EMSA, DNA Footprinting & Microarrays

Animal Tissue Culture and Hybridoma Technology

6 Topics
Hybridoma Technology & Monoclonal Antibodies: PEG Cell Fusion & HAT Selection Mechanics (HGPRT/TK) Cellular Immunotherapies: CAR-T Cell Engineering & Therapeutic Antibody Production Flow Cytometry, Fluorescence-Activated Cell Sorting (FACS) & Cell Cycle DNA Analysis Primary Cell Culture vs Established Cell Lines: Media Formulation, Sera & Subculturing Protocols Bioreactors, Microcarriers, Scale-Up & Suspension Cloning for Selective Tumor Cell Culture Cryopreservation of Cells: Cryoprotectants (DMSO, Glycerol), Controlled Freezing & Thawing Kinetics Active

Muscle Physiology

1 Topic
Muscle Energetics, Fiber Types (Red vs White), Rigor Mortis & Muscle Fatigue

Embryo Transfer and Related Techniques

5 Topics
Somatic Cell Nuclear Transfer (SCNT), Microinjection & Animal Cloning Milestones Superovulation Hormonal Regimes in Bovine & Rodents (PMSG, FSH, LH, hCG) In Vitro Fertilization (IVF), Oocyte Maturation & Evidence of Fertilization in Stained Ova Embryo Sexing Technologies: PCR Amplification of Y-Specific Repeats & LAMP Assays Transgenic Animal Production: Microinjection, Retro-viral Vectors, Stem Cells & Gene Farming

Nervous System

1 Topic
Motor Control & Cerebellar Coordination: Cerebellum as Error-Correcting Device

Reproduction and Lactation

2 Topics
Male Reproductive Physiology: Spermatogenesis, Sertoli Cells & Pheromones Female Reproductive Cyclicity: Two vs Three Wave Estrous Cycles & Ovulation

Climate Physiology

1 Topic
Climatic Heat Stress in Domestic Livestock: Heat Tolerance Indices at 65% RH
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