Q.1
Most commonly used mounting media for viewing fungi is
- Mounting Media in Diagnostic Mycology: The most widely utilized universal mounting and staining reagent for temporary microscopic mounts of fungal mycelia, conidiophores, and spores is Cotton blue in lactophenol (LPCB).
- Chemical Constituents and Functions:
- Phenol: Rapidly kills fungal hyphae and inactivates autolytic enzymes.
- Lactic acid: Preserves fungal cellular morphology and structures.
- Glycerol: Maintains an osmotic balance and prevents the mount from drying out.
- Cotton blue (Aniline blue): Acidic stain that binds intensely to fungal cell-wall chitin, staining hyphae and reproductive spores bright blue.
- Conclusion: Cotton blue in lactophenol is the standard mounting medium (Option 2).
Correct option: (2) Cotton blue in lacto-phenol
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