Q.1
Why is actin used as a control for transcriptome studies in vertebrates?
- Endogenous Controls in Quantitative RT-PCR and Transcriptomics:
- Actin ($eta$-actin / ACTB): Encodes a ubiquitous cytoskeletal microfilament protein required for structural cell maintenance.
- It is utilized as a standard positive internal reference (housekeeping control) in Northern blots, RT-qPCR, and transcriptome assays because its basal mRNA expression level is fairly constant and stable across different cell types, tissues, and experimental physiological conditions.
- nalysis:
- (It is used as a positive control as actin expression is fairly constant in different cell types): Correct.
Correct option: (3) It is used as a positive control as actin expression is fairly constant in different cell types.
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