Q.116
Which of the following tools can be used to study the distribution of bacterial community in a sample?
(A). 16S rRNA PCR
(B). Amplicon sequencing
(C). DGGE
(0). RAPD
Choose the correct answer from the options given below:
- Culture-Independent Molecular Community Profiling:
- High-throughput Amplicon Sequencing: Directly reveals the full taxonomic profile and relative abundance of bacterial populations in food and environmental samples.
- Denaturing Gradient Gel Electrophoresis (DGGE): Separates identical-length 16S rRNA PCR amplicons along a denaturing gradient gel to visualize community band patterns and shifts.
- Conclusion: (B) and (C) only (Option 3).
Correct option: (3) (B) and (C) only.
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