Q.110
Which of the following is not a PCR based molecular marker?
- Classification of Molecular Markers:
- Non-PCR Based (Hybridization-Based) Markers:
- RFLP (Restriction Fragment Length Polymorphism): Depends on genomic DNA digestion with restriction endonucleases followed by Southern blotting and probe hybridization. It does not employ PCR amplification.
- PCR-Based Molecular Markers:
- SSR (Simple Sequence Repeats / Microsatellites): Amplified using sequence-specific locus primers.
- AFLP (Amplified Fragment Length Polymorphism): Uses adapter ligation followed by selective PCR amplification.
- SNP (Single Nucleotide Polymorphism): Genotyped using PCR-based sequencing, KASP, or high-density SNP arrays.
- Correct Option: Option 4 (RFLP).
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