Q.21
Which of the following marker system is called as Selective Restriction Fragment Amplification?
EXPLANATION
Molecular Markers & Genetic Diversity Analysis
- AFLP Marker System: Amplified Fragment Length Polymorphism (AFLP), developed by Vos et al. (1995), is also formally referred to as Selective Restriction Fragment Amplification (SRFA).
- Principle: Digestion of genomic DNA with restriction enzymes (typically EcoRI and MseI), ligation of double-stranded adaptors, and selective PCR amplification using primers containing 1 to 3 selective nucleotides. (Correct answer: Option 2)
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