Q.25
Livestock Production Management- ICAR JRF/SRF 2024
MCQ (Single Correct) +4 −1

Which of the following enzyme degrades unincorporated nucleotides and excess ATP in pyrosequencing method

EXPLANATION Recombinant DNA Technology, PCR, DNA Sequencing, Cloning and Transgenic Animals
  • Enzymatic Cascade of Pyrosequencing (4-Enzyme System):
  • DNA Polymerase: Incorporates complementary dNTP, releasing pyrophosphate ($PP_i$).
  • ATP Sulfurylase: Converts $PP_i$ and adenosine $5'$-phosphosulfate (APS) into ATP.
  • Luciferase: Utilizes ATP to oxidize luciferin into oxyluciferin, generating a visible light pulse proportional to incorporated bases.
  • Apyrase: An ATP-diphosphohydrolase that rapidly degrades unincorporated dNTPs and residual ATP between reagent cycles, preventing false background signals before the next nucleotide is introduced.
    • nalysis:

  • (Apyrase): Correct nucleotide-degrading enzyme.
Correct option: (4) Apyrase

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