Q.74
A temperate, dsDNA bacteriophage which replicates by transposition is
- Bacteriophage Mu (Mutator Phage): Bacteriophage Mu is a temperate double-stranded DNA bacteriophage that infects E. coli. It is unique because it replicates entirely by replicative transposition.
- Mutator Phenotype: During both lysogenic integration and lytic amplification, Mu inserts its $37\text{ kb}$ genome essentially at random positions throughout the host chromosome via transposase (MuA) and accessory protein (MuB), causing high-frequency insertion mutations ($1-3\%$ of host genes inactivated, hence named 'Mu' for mutator).
- Other Phages:
- Lambda ($\lambda$): Replicates via bidirectional theta replication followed by rolling circle replication, integrating via site-specific recombination at attB.
- T7: Virulent linear dsDNA phage replicating by concatemer formation.
- M13: Filamentous ssDNA phage replicating by rolling circle mechanism without transposition.
- Conclusion: Phage Mu replicates by transposition (Option 3).
Correct option: (3) Mu
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