Q.82
The organism which contains genes encoding Mo and V nitrogenases as well as Fe-only nitrogenase is
- Nitrogenase Isoforms in Methanogenic Archaea: Biological nitrogen fixation is catalyzed by nitrogenase enzyme complexes. While most diazotrophs contain only molybdenum nitrogenase (Mo-nitrogenase, encoded by nifHDK), some diazotrophs possess alternative nitrogenases:
- Molybdenum-dependent nitrogenase (nif)
- Vanadium-dependent nitrogenase (vnf)
- Iron-only nitrogenase (anf)
- Organism Identification: The methanogenic archaeon Methanosarcina barkeri is genetically unique in possessing all three nitrogenase systems (Mo-nitrogenase, V-nitrogenase, and Fe-only nitrogenase), allowing it to fix dinitrogen across diverse trace metal regimes.
- Conclusion: Methanosarcina barkeri contains genes encoding Mo, V, and Fe-only nitrogenases (Option 2).
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