Plant Genetic Resources- ICAR JRF/SRF 2024

• Wright's Fixation Index ($F_{ST}$) Thresholds:
• Sewall Wright established benchmark guidelines for interpreting genetic differentiation ($F_{ST}$):
• $0.00 \le F_{ST} \lt 0.05$: Little genetic differentiation
• $0.05 \le F_{ST} \lt 0.15$: Moderate genetic differentiation
• $0.15 \le F_{ST} \le 0.25$: Great genetic differentiation
• $F_{ST} \gt 0.25$: Very great genetic differentiation
• An estimate of $F_{ST}$ just below $0.25$ indicates Great genetic differentiation.
• Conclusion: Great genetic differentiation (Option 3).

Correct option: (3) Great genetic differentiation

• Distinguishing Feature of QTL-seq and BSA-seq:
• Conventional QTL mapping requires individual genotyping of hundreds of individual plants across a mapping population.
• In contrast, QTL-seq and BSA-seq sequence two pooled bulks of progeny exhibiting contrasting extreme phenotypes (Option A), identifying candidate loci based on allele frequency divergence without individual genotyping.
• Conclusion: Option A only (Option 1).

Correct option: (1) Option A only

• PCR-Based Transgene Detection:
• Standard PCR (target amplification) and Real-Time PCR (SYBR Green / TaqMan assays) are deployed in quarantine molecular laboratories specifically for qualitative and quantitative detection of the physical presence of transgenes (foreign DNA construct sequences) in imported germplasm lots.
• Conclusion: Only Option A is correct (Option 1).