Q.77
Veterinary Public Health and Epidemiology- ICAR JRF/SRF 2024
MCQ (Single Correct)

Which among the following statements are correct for "Direct Microscopic Count” (DMC) of milk?

(A) . Itis a long procedure and thus is not used as a platform test for milk.
(B) . It can not differentiate between live and dead bacterial cells.
(C) . In this test, staining is done with Newman stain.
(D) . Breed's pipette is used for this test

Choose the correct answer from the options given below:

  • Principles of Direct Microscopic Count (DMC):
  • Platform Test Utility: DMC is a very rapid platform test (takes only $10\text{--}15\text{ minutes}$) widely used in dairy reception docks for rapid screening, NOT a long procedure [Statement A is FALSE].
  • Viability Discrimination: Direct microscopic counting cannot differentiate between viable (living) and dead bacterial cells, as both take up the basic dye [Statement B is TRUE].
  • Newman-Lampert Stain: Newman's modified stain (containing methylene blue, ethyl alcohol, tetrachloroethane, and glacial acetic acid) is used to simultaneously defat, fix, and stain milk smears [Statement C is TRUE].
  • Breed's Pipette: A calibrated Breed pipette delivers precisely $0.01\text{ ml}$ of milk over a calibrated $1\text{ cm}^2$ area on a glass slide to calculate the microscopic factor (MF) [Statement D is TRUE].

Correct option: (3) (B), (C) and (D) only.

Explanation not matching this question? क्या व्याख्या इस प्रश्न से मेल नहीं खा रही? Report
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