• Detection of Bacterial Enterotoxins: Extracellular bacterial enterotoxins released into culture media (e.g., staphylococcal enterotoxins SEA-SEE, Bacillus cereus enterotoxin) can be detected using diverse immunological and biological methods:
• ELISA: Sandwich enzyme-linked immunosorbent assay provides high analytical sensitivity ($\lt 1\text{ ng/ml}$) for screening enterotoxins.
• Bioassays: Traditional functional assays including the ligated rabbit ileal loop (RIL) model, suckling mouse assay, and monkey feeding assay.
• Microslide Double Diffusion: Classical Wadsworth microslide gel double diffusion technique for enterotoxin serotyping.
• Radial Immunoassay / Immunodiffusion: Mancini single radial immunodiffusion (SRID) and reverse passive latex agglutination (RPLA).

Correct option: (1) (A), (B), (C) and (D).

Matching the items of List I with List II for Fish and meat from game animals:

The correct combination is Option 1: (A) - (1), (B)- (IV), (C)- (N), (D) - (I).

Correct option: (1) (A) - (1), (B)- (IV), (C)- (N), (D) - (I)

• Origin of HACCP: The Hazard Analysis Critical Control Point (HACCP) system was developed in the late 1960s by Dr. Howard E. Bauman and colleagues at the Pillsbury Company, working collaboratively with NASA and the U.S. Army Natick Laboratories.
• Original Objective: Designed to provide 100% assurance of food safety and eliminate the risk of foodborne illnesses during manned Apollo space missions.
• Other Pioneers Listed:
• Franz von Soxhlet: Pioneer of chemical Soxhlet extraction apparatus and milk pasteurization.
• W. Edwards Deming: Father of modern Total Quality Management (TQM) and the Deming PDCA Cycle.

Correct option: (1) H.E.Bauman