Which term is commonly used to describe the specific sequence of bases recognized by restriction enzymes?
प्रतिबंधन एंजाइमों (restriction enzymes) द्वारा पहचाने जाने वाले क्षारों के विशिष्ट अनुक्रम का वर्णन करने के लिए आमतौर पर किस शब्द का उपयोग किया जाता है?
Restriction Endonucleases and Recognition Sequences:
- Restriction endonucleases (molecular scissors) cleave double-stranded DNA at or near specific nucleotide sequences.
- The specific 4 to 8 base-pair DNA sequence recognized and bound by a restriction endonuclease is officially known as the Recognition sequence or Restriction site.
- In Type II restriction enzymes (such as EcoRI, recognizing $5'\text{-GAATTC-}3'$), these sites are typically palindromic sequences with two-fold rotational symmetry.
Correct Answer: Option 3 (Recognition sequence or restriction site).
विस्तृत व्याख्या:
Restriction Endonucleases and Recognition Sequences (प्रतिबंधन स्थल):
- Restriction endonucleases (molecular scissors) cleave double-stranded DNA at or near specific nucleotide sequences.
- The specific 4 to 8 base-pair DNA sequence recognized and bound by a restriction endonuclease is officially known as the Recognition sequence or Restriction site (पहचान अनुक्रम या प्रतिबंधन स्थल).
- In Type II restriction enzymes (such as EcoRI, recognizing $5'\text{-GAATTC-}3'$), these sites are typically palindromic sequences with two-fold rotational symmetry.
Correct Answer: Option 3 (Recognition sequence or restriction site).
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