Secondary Metabolite Classes: Alkaloids, Terpenoids, Phenolics, Flavonoids, Saponins, Lignans (Enterodiol) & Glucosinolates (Sinigrin)

• Fundamental Principles of Chromatography:
• Theoretical Plate ($N$): Measure of peak broadening and column efficiency ($N = 16(t_r/W)^2$) $\rightarrow$ (I)
• Stationary Phase: Immobile phase (silica, bonded alkyl phase) that retards analytes in chromatography $\rightarrow$ (II)
• Resolution ($R_s$): Quantitative degree of physical separation between two adjacent chromatographic peaks $\rightarrow$ (III)
• Retardation Factor ($R_f$): Ratio of distance traveled by solute to that by solvent front in Thin Layer Chromatography (TLC) $\rightarrow$ (IV)
• Systematic Match: Corresponds to Option 3.

Correct option: (3) (A) - (IV), (B) - (I), (C) - (I), (B) - (1)