In serological diagnostics, the Complement Fixation Test (CFT) utilizes antigen-antibody complex formation to fix (consume) free complement components.
It measures the presence and antigenic titer (amount) of specific viral or bacterial antigens or antibodies. It does not assess the viability or infectivity of the pathogen.
Correct Option: Option 1 (the presence and amount of virus antigen).
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Aquatic Animal Health Management- ICAR JRF/SRF 2024 (Q.101)
MCQ (Single Correct)+4−1
Hybridoma are grown in which of the selective medium?
In monoclonal antibody production, splenic B lymphocytes are fused with myeloma cells deficient in Hypoxanthine-Guanine Phosphoribosyltransferase ($HGPRT^-$).
Unfused myeloma cells and non-functional hybrids are eliminated by culture in HAT Medium (Hypoxanthine, Aminopterin, Thymidine):
Aminopterin blocks the de novo nucleotide synthesis pathway.
Only fused hybridomas possessing functional HGPRT from the B cell survive by utilizing the salvage pathway.
Correct Option: Option 1 (HAT medium).
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