1
Which of the following technique is most frequently used for culture preservation in microbial culture collection centers?
EXPLANATION
Culture Media Formulations, Pure Culture Isol
- Lyophilization (Freeze-Drying): The standard and most frequently utilized preservation method in international microbial culture collections (such as ATCC, MTCC, DSMZ).
- Process & Advantages: Involves flash-freezing the cell suspension followed by primary and secondary sublimation of ice under high vacuum. It preserves bacterial, actinomycete, and fungal viability and genetic stability for decades (20–30+ years) in hermetically sealed glass ampoules without subculturing.
Correct option: (3) Lyophilization
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