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Which of the following restriction endonuclease pairs are isochizomers?
EXPLANATION
Recombinant DNA Technology, Cloning Vectors,
- Definition of Isoschizomers: Restriction endonucleases that recognize the identical target nucleotide sequence.
- Hpall and Mspl: Both enzymes recognize the tetranucleotide palindromic sequence $5'\text{-CCGG-3'}$. They differ in methylation sensitivity: HpaII is inhibited if the internal cytosine is methylated ($5'\text{-C}^m\text{CGG-3'}$), whereas MspI cleaves whether the internal cytosine is methylated or unmethylated, making them indispensable tools for studying DNA methylation.
- Neoschizomers: Enzymes that recognize the same sequence but cleave at different positions.
Correct option: (2) Hpall and Mspl
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