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Which technique is used to detect specific DNA molecule ?
EXPLANATION
Recombinant DNA Technology, Cloning Vectors,
- Southern Blotting: Developed by British biologist Edwin Southern (1975) to identify and detect specific target DNA molecules.
- Process: DNA is digested with restriction endonucleases, resolved by agarose gel electrophoresis, capillary-transferred to a nitrocellulose or nylon membrane, and hybridized with a labeled complementary DNA/RNA probe.
- Distinctions: Northern blotting detects specific RNA; Western blotting detects specific proteins; Eastern blotting detects post-translational modifications.
Correct option: (1) Southem blotting
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