Q.1
Biochemistry- ICAR JRF/SRF 2024 (Q.47)
MCQ (Single Correct) +4 −1

Which of the following genes are genetically engineered in biofortified GOLDEN RICE?

  • Golden Rice Engineering (Ingo Potrykus & Peter Beyer): Genetically engineered to synthesize provitamin A ($\beta$-carotene) in the rice endosperm by introducing two key biosynthetic genes:
  • psy (Phytoene Synthase) from daffodil (Narcissus pseudonarcissus) or maize (Zea mays), converting geranylgeranyl diphosphate (GGPP) to phytoene.
  • crtI (Phytoene Desaturase / Carotene Desaturase) from the bacterium Pantoea ananatis (Erwinia uredovora), performing multiple desaturation steps to yield lycopene.
    • Conclusion: The engineered genes are Phytoene synthase and Phytoene Desaturase.

Correct option: (3) Phytoene synthase and Phytoene Desaturase

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